Nicotra A, Schatten G
Department of Animal and Human Biology, University of Rome, Italy.
Mol Reprod Dev. 1996 Mar;43(3):387-91. doi: 10.1002/(SICI)1098-2795(199603)43:3<387::AID-MRD13>3.0.CO;2-Z.
Propranolol, a beta-adrenergic receptor blocker, is found to induce polyspermy in sea urchin eggs. Unfertilized sea urchin eggs treated for 10 min with 50 microM of propranolol, and then inseminated, become polyspermic and show a fertilization envelope which is barely visible to the light microscope. Examination of treated eggs by transmission and scanning electron microscopy shows that the drug does not alter the cortex of the unfertilized egg. However, after insemination an incomplete cortical reaction occurs. This might well account for both polyspermy and the defective elevation of the fertilization envelope. Since the effects of the drug are reversed by simultaneous treatment with adrenalin, perhaps propranolol interferes with the monoaminergic system that has been proposed to be active. The involvement of the monoaminergic system in the fertilization process is present in the sea urchin egg.
普萘洛尔是一种β-肾上腺素能受体阻滞剂，已发现它会诱导海胆卵发生多精入卵现象。用50微摩尔的普萘洛尔处理未受精的海胆卵10分钟，然后进行授精，这些卵会变成多精入卵，并且出现一个在光学显微镜下几乎看不见的受精膜。通过透射电子显微镜和扫描电子显微镜对处理过的卵进行检查表明，该药物不会改变未受精卵的皮质。然而，授精后会发生不完全的皮质反应。这很可能是多精入卵和受精膜升高缺陷的原因。由于同时用肾上腺素处理可逆转该药物的作用，或许普萘洛尔会干扰已被认为具有活性的单胺能系统。单胺能系统参与海胆卵的受精过程。
