Hu J C, Zhang C, Slavkin H C
Department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio, 78284-7888, USA.
Int J Dev Biol. 1995 Dec;39(6):939-45.
Platelet-derived growth factor (PDGF) is a potent mitogen that functions in cytodifferentiation and wound healing. PDGF receptor-alpha (PDGFR-alpha) is required for the normal development of the dental ectomesenchyme (Stephenson et al., Proc. Natl. Acad. Sci. USA 88: 6-10, 1991). To investigate the regulatory potential of PDGF on tooth development, the expression of PDGF-AA was determined by reverse transcription-polymerase chain reaction (RT-PCR), the PDGF ligands and receptors were localized by immunohistochemistry. The growth-promoting effects of exogenous PDGF-AA on molar explants were determined by assaying for tritiated thymidine incorporation, the presence of type I collagen and amelogenin messenger RNA transcripts, and total DNA, RNA and protein accumulation. Based upon the temporal and spatial localization, PDGFs and their receptors are present in the enamel epithelia and pulpal mesenchyme of developing mouse molars. Exogenous PDGF-AA administration increased the total protein accumulation of mouse molar explants but produces no discernible effect on amelogenin and type I collagen expression.
血小板衍生生长因子（PDGF）是一种在细胞分化和伤口愈合中起作用的强效有丝分裂原。血小板衍生生长因子受体α（PDGFR-α）是牙齿外胚间充质正常发育所必需的（斯蒂芬森等人，《美国国家科学院院刊》88: 6 - 10，1991）。为了研究PDGF对牙齿发育的调控潜力，通过逆转录聚合酶链反应（RT-PCR）测定PDGF-AA的表达，通过免疫组织化学对PDGF配体和受体进行定位。通过检测氚标记胸腺嘧啶核苷掺入、I型胶原和釉原蛋白信使RNA转录本的存在以及总DNA、RNA和蛋白质积累，确定外源性PDGF-AA对磨牙外植体的促生长作用。基于时间和空间定位，PDGF及其受体存在于发育中小鼠磨牙的釉质上皮和牙髓间充质中。给予外源性PDGF-AA可增加小鼠磨牙外植体的总蛋白积累，但对釉原蛋白和I型胶原表达没有明显影响。
