Terling C, Rass A, Mitsiadis T A, Fried K, Lendahl U, Wroblewski J
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
Int J Dev Biol. 1995 Dec;39(6):947-56.
The developing tooth represents a suitable model for understanding the molecular mechanisms involved in induction, morphogenesis and differentiation of organs. It is conceivable that the developmental changes could be reflected in the distribution of different cytoskeletal components and in this report we analyze the expression of the intermediate filament nestin during rodent tooth development at the protein and mRNA levels (by immuno light and electron microscopy, and by in situ hybridization). Nestin is expressed at all stages of tooth development, but the expression levels increase after birth in both ectodermal and ectomesenchymal derivatives. The shift in nestin distribution, from the proliferating dental lamina to the dental mesenchyme, indicates that nestin may be involved in inductive phenomena. At early stages of mineralization, nestin is seen within the apical parts of the presecretory ameloblasts. Nestin is also expressed in odontoblasts, both during odontogenesis and after tooth eruption. The increase in nestin expression from early to late developmental stages and sustained expression in a differentiated cell type contrasts with previously observed patterns of nestin expression during nerve and muscle development. This suggests that nestin could be used as a specific marker for the odontoblast.
发育中的牙齿是理解器官诱导、形态发生和分化所涉及分子机制的合适模型。可以想象，发育变化可能反映在不同细胞骨架成分的分布上，在本报告中，我们在蛋白质和mRNA水平（通过免疫光镜和电镜以及原位杂交）分析了啮齿动物牙齿发育过程中中间丝巢蛋白的表达。巢蛋白在牙齿发育的所有阶段均有表达，但出生后在外胚层和外间充质衍生物中的表达水平均升高。巢蛋白分布从增殖的牙板向牙间充质的转变表明巢蛋白可能参与诱导现象。在矿化早期，在分泌前期成釉细胞的顶端部分可见巢蛋白。在牙本质形成过程中和牙齿萌出后，成牙本质细胞中也表达巢蛋白。从发育早期到晚期巢蛋白表达增加，并在分化细胞类型中持续表达，这与之前在神经和肌肉发育过程中观察到的巢蛋白表达模式不同。这表明巢蛋白可作为成牙本质细胞的特异性标志物。
