Elbakidze G M, Livanova L M
Biull Eksp Biol Med. 1977 Jul;84(7):32-5.
The influence of endogenous uncoupling agent from the rat liver on oxidative phosphorylation of the liver, kidney, heart, lungs, and brain mitochondria was studied; the tissue-specificity of its action was demonstrated. The selective action of the uncoupler on the mitochondrial membrane is more obviously expressed within the range of pH 6.9--6.3 approaching such of the hepatocyte cytoplasm. The tissue-specificity was independent of the original state of mitochondria and could be eliminated only by heat injury of the oxidative phosphorylation. The activity of the tissue-specific uncoupling factor failed to diminish when the ribosomes and microsomes were removed by centrifugation. Addition of bovine albumin to the incubation medium had no influence of the value of the tissue-specific uncoupling. Participation of the uncoupler in the intratissue proliferation control is discussed.
研究了大鼠肝脏内源性解偶联剂对肝脏、肾脏、心脏、肺和脑线粒体氧化磷酸化的影响；证明了其作用的组织特异性。在接近肝细胞胞质pH值6.9 - 6.3的范围内，解偶联剂对线粒体膜的选择性作用更为明显地表现出来。这种组织特异性与线粒体的原始状态无关，只有通过氧化磷酸化的热损伤才能消除。当通过离心去除核糖体和微粒体时，组织特异性解偶联因子的活性并未降低。向孵育培养基中添加牛血清白蛋白对组织特异性解偶联的值没有影响。讨论了解偶联剂在组织内增殖控制中的作用。
