大鼠不同组织线粒体中NADH和琥珀酸脱氢酶位点的亲脂性和呼吸特性。
Lipophilic and respiratory properties of NADH and succinate dehydrogenase sites in mitochondria from various tissues of the rat.
作者信息
Higgins E S, Rogers K S
出版信息
Proc Soc Exp Biol Med. 1976 Jun;152(2):221-3. doi: 10.3181/00379727-152-39365.
Mitochondria were isolated from heart, liver, kidney, spleen, and brain of the rat. With overall regard to both resting and activated respiratory velocities with either glutamate or succinate, as well as the respective degrees of respiratory control, kidney mitochondria were most efficient and spleen mitochondria least so. A probe of mitochondrial inner membrane lipophilicity with tetrabutylammonium bromide showed that NADH dehydrogenases from liver and heart were similar, as were also those from kidney and spleen. With the exception of brain, only small differences were observed in lipophilic properties of succinate dehydrogenases from the various other tissues. Variation in lipophilic characteristics of the two sites on the mitochondrial inner membranes could not be correlated with embryological origin of the tissue.
从大鼠的心脏、肝脏、肾脏、脾脏和大脑中分离出线粒体。综合考虑用谷氨酸或琥珀酸时的静息呼吸速度和激活呼吸速度，以及各自的呼吸控制程度，肾脏线粒体效率最高，脾脏线粒体效率最低。用溴化四丁铵对线粒体内膜亲脂性进行检测表明，肝脏和心脏的NADH脱氢酶相似，肾脏和脾脏的也相似。除大脑外，其他各种组织的琥珀酸脱氢酶的亲脂特性仅观察到微小差异。线粒体内膜上这两个位点亲脂特性的变化与组织的胚胎起源无关。
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