Legastelois I, Cordier G, Cozon G, Cadoré J L, Guiguen F, Greenland T, Mornex J F
Laboratoire d'Immunologie et de Biologie Pulmonaire, INSERM CJF 93-08, Université Claude Bernard, Lyon, France.
Res Virol. 1996 Mar-Jun;147(2-3):191-7. doi: 10.1016/0923-2516(96)80234-2.
Visna-maedi virus is a lentivirus which causes inflammatory disorders in sheep, including a chronic interstitial lung disease resembling that observed in human immunodeficiency virus type 1 (HIV 1) infection. In view of our previous demonstration of the production of neutrophil chemotactic activity by alveolar macrophages, and given the lymphocytic and neutrophilic nature of the alveolar cell infiltrate in both naturally and experimentally infected animals, we hypothesized that interleukin-8 (IL8) could be a candidate for at least part of the chemotactic activity we described. In this study, we investigated IL8 mRNA expression following visna-maedi virus infection. Northern analysis of total RNA using an ovine IL8-specific probe demonstrated that the IL8 gene is upregulated in alveolar macrophages as a consequence of in vitro infection and in alveolar cells from experimentally infected animals. Using a semi-quantitative RT-PCR method, we showed that various levels of IL8 mRNA are expressed by alveolar cells from infected animals and that they correlate with the intensity of the lesions. In conclusion, visna-maedi virus is able to induce IL8 mRNA expression in sheep alveolar cells. Results from in vivo infected animals suggest that IL8 could play a role in the early build-up of visna-maedi virus-induced lesions.
维斯纳-梅迪病毒是一种慢病毒，可引起绵羊的炎症性疾病，包括一种类似于人类1型免疫缺陷病毒（HIV-1）感染中观察到的慢性间质性肺病。鉴于我们之前证明肺泡巨噬细胞可产生中性粒细胞趋化活性，并且考虑到自然感染和实验感染动物肺泡细胞浸润的淋巴细胞和中性粒细胞性质，我们推测白细胞介素-8（IL-8）可能是我们所描述的趋化活性至少一部分的候选因素。在本研究中，我们调查了维斯纳-梅迪病毒感染后IL-8 mRNA的表达。使用绵羊IL-8特异性探针对总RNA进行Northern分析表明，由于体外感染以及实验感染动物的肺泡细胞中，IL-8基因在肺泡巨噬细胞中上调。使用半定量RT-PCR方法，我们表明感染动物的肺泡细胞表达不同水平的IL-8 mRNA，并且它们与病变强度相关。总之，维斯纳-梅迪病毒能够诱导绵羊肺泡细胞中IL-8 mRNA的表达。体内感染动物的结果表明，IL-8可能在维斯纳-梅迪病毒诱导的病变早期形成中起作用。
