低水平吸入暴露后啮齿动物体内1,3 - 丁二烯代谢生成丁二烯单环氧化物和丁二烯二环氧化物的性别及物种差异。

Gender and species differences in the metabolism of 1,3-butadiene to butadiene monoepoxide and butadiene diepoxide in rodents following low-level inhalation exposures.

作者信息

Thornton-Manning J R, Dahl A R, Bechtold W E, Henderson R F

机构信息

Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185, USA.

出版信息

Toxicology. 1996 Oct 28;113(1-3):322-5. doi: 10.1016/0300-483x(96)03466-x.

DOI:10.1016/0300-483x(96)03466-x

PMID:8901918

Abstract

Levels of butadiene monoepoxide (BDO) and butadiene diepoxide (BDO2) were compared in tissues of male Sprague-Dawley rats and male B6C3F1 mice and in tissues of male and female Sprague-Dawley rats following inhalation exposures to 62.5 ppm 1,3-butadiene (BD). In male rats, BDO2 levels were highest in blood and were present at a concentration of only 5 +/- 1 pmol/g. Following a 6-h exposure, the concentration of BDO2 in the blood, femurs, lung and fat of female rats was 3 to 7-fold that of male rats. Levels of BDO were similar in tissues of female and male rats. Generally, levels of BDO were approximately 3 to 8-fold greater in mouse tissues as compared with rat tissues following 4-h exposures to BD. In blood, 204 +/- 15 pmol/g BDO2 was detected in male mice, while in rats, blood BDO2 levels were 5 +/- 1 pmol/g. This study shows marked species differences in tissue levels of BD epoxides, particularly BDO2, in rats and mice, and is the first to show gender differences in BD metabolism.

摘要

在雄性斯普拉格-道利大鼠和雄性B6C3F1小鼠的组织中，以及在雄性和雌性斯普拉格-道利大鼠吸入62.5 ppm 1,3 - 丁二烯（BD）后的组织中，比较了丁二烯单环氧化物（BDO）和丁二烯二环氧化物（BDO2）的水平。在雄性大鼠中，BDO2水平在血液中最高，其浓度仅为5±1 pmol/g。暴露6小时后，雌性大鼠血液、股骨、肺和脂肪中BDO2的浓度是雄性大鼠的3至7倍。雌性和雄性大鼠组织中的BDO水平相似。一般来说，在暴露于BD 4小时后，小鼠组织中BDO的水平比大鼠组织高约3至8倍。在血液中，雄性小鼠检测到204±15 pmol/g的BDO2，而在大鼠中，血液BDO2水平为5±1 pmol/g。这项研究表明，大鼠和小鼠组织中BD环氧化物水平，特别是BDO2，存在明显的物种差异，并且首次表明BD代谢存在性别差异。