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Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies.

作者信息

Thompson N E, Burgess R R

机构信息

McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706, USA.

出版信息

Methods Enzymol. 1996;274:513-26. doi: 10.1016/s0076-6879(96)74041-7.

DOI:10.1016/s0076-6879(96)74041-7
PMID:8902829
Abstract
摘要

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Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies.使用多元醇反应性单克隆抗体对RNA聚合酶II和转录因子进行免疫亲和纯化。
Methods Enzymol. 1996;274:513-26. doi: 10.1016/s0076-6879(96)74041-7.
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Purification of human RNA polymerase II and general transcription factors.人RNA聚合酶II和通用转录因子的纯化。
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Affinity purification of a human RNA polymerase II complex using monoclonal antibodies against transcription factor IIF.使用抗转录因子IIF单克隆抗体对人RNA聚合酶II复合物进行亲和纯化。
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Activator-dependent transcription by mammalian RNA polymerase II: in vitro reconstitution with general transcription factors and cofactors.哺乳动物RNA聚合酶II依赖激活因子的转录:利用通用转录因子和辅因子进行体外重建。
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