Mogensen P K, Møller J, Nyborg L, Magid E, Wimmelmann N, Uldall A
Department of Clinical Chemistry, Herning Centralsygehus, Denmark.
Scand J Clin Lab Invest. 1996 Oct;56(6):505-9. doi: 10.3109/00365519609088806.
In a multilaboratory study a consensus was established to calibrate the Ektachem Amylase method to reproduce the results of the Phadebas Blue Starch method. The calibration graph has a slope = 3.39 and intercept = 25. For the Ektachem Amylase method the reaction-rate ratio between salivary and pancreatic amylase was calculated to be 0.89, relative to that of the Phadebas Blue Starch method. A calibration value for the Nordic Amylase Calibrator to be used on Ektachem analysers was determined to be close to 469 U l-1 for the current batch. However, since the difference from the stated value of 460 U l-1 is negligible, the authors recommend the use of the stated value for this and future batches of the Nordic Amylase Calibrator. An error of around 10% introduced by the presence of salivary amylase is comparable to the methods accepted by the Scandinavian Committee on Enzymes. Introduction of a consensus calibration reduced the interlaboratory variation by up to 40% at all levels.
在一项多实验室研究中，达成了一项共识，即校准Ektachem淀粉酶法，以重现Phadebas蓝淀粉法的结果。校准图的斜率为3.39，截距为25。对于Ektachem淀粉酶法，相对于Phadebas蓝淀粉法，唾液淀粉酶与胰淀粉酶之间的反应速率比经计算为0.89。已确定用于Ektachem分析仪的北欧淀粉酶校准品的当前批次校准值接近469 U l-1。然而，由于与规定值460 U l-1的差异可忽略不计，作者建议对该批次及以后批次的北欧淀粉酶校准品使用规定值。唾液淀粉酶的存在引入的约10%的误差与斯堪的纳维亚酶委员会认可的方法相当。采用共识校准可将所有水平的实验室间差异降低多达40%。
