Gerhardt W, Waldenström J, Hörder M, Magid E, Strömme J H, Theodorsen L, Härkönen M, Icén A
Scand J Clin Lab Invest. 1986 Sep;46(5):465-9. doi: 10.3109/00365518609083699.
Eighty-seven Nordic Hospital laboratories participated in a joint SCE-NORDKEM follow-up study of the long-term stability of the previously established calibration factors for a number of alpha-amylase routine methods based on six different substrates. Human control materials with 90% pancreatic, 90% salivary, and pure pancreatic alpha-amylases were measured by the participants. The data were plotted before and after calibration of each method using a human pancreatic calibrator with an assigned catalytic concentration of 390 U/l (Phadebas blue starch method, 37 degrees C). As in the previous study, carried out 9 months earlier, the pre-calibration values varied over a six-fold range. The post-calibration values of all methods except those based on a tetraose substrate showed an acceptable inter-laboratory comparability. As a temporary measure, SCE recommends that the Nordic laboratories calibrate the accepted routine methods by their individual calibration factor. Detailed suggestions for calibration procedures and a discussion of the principles of transferability will shortly be published by the SCE in this journal.
87家北欧医院实验室参与了一项由临床化学欧洲学会-北欧临床化学协会（SCE-NORDKEM）联合开展的随访研究，该研究针对此前基于六种不同底物所确立的多种α-淀粉酶常规方法校准因子的长期稳定性。参与人员测定了含90%胰腺α-淀粉酶、90%唾液α-淀粉酶及纯胰腺α-淀粉酶的人源对照物质。使用催化浓度设定为390 U/l的人源胰腺校准物（Phadebas蓝淀粉法，37摄氏度），在各方法校准前后绘制数据图。与9个月前开展的上一项研究一样，校准前的值在六倍范围内波动。除基于四糖底物的方法外，所有方法校准后的数值均显示出可接受的实验室间可比性。作为一项临时措施，SCE建议北欧实验室通过各自的校准因子对公认的常规方法进行校准。SCE将很快在本期刊上发表校准程序的详细建议以及可转移性原理的讨论。
