Kim J D, Yu B P, McCarter R J, Lee S Y, Herlihy J T
Department of Physiology, University of Texas Health Science Center at San Antonio, USA.
Free Radic Biol Med. 1996;20(1):83-8. doi: 10.1016/0891-5849(95)02023-3.
Free radical metabolism can be altered by several interventions, including dietary restriction (DR) and exercise. Most of the previous work has focused on the liver and skeletal muscle. The following experiments were performed to determine whether long-term DR and chronic exercise affect free radical metabolism and change the status of the antioxidant defenses of the heart. Rats were subjected to DR and/or endurance exercise for 18.5 months and were sacrificed along with their ad lib fed and sedentary controls. Both DR and exercise decreased the malondialdehyde content of cardiac mitochondria, indicating a decrease in lipid peroxidation damage. The antioxidant enzymes in the cytosol, superoxide dismutase, selenium dependent glutathione peroxidase, and glutathione S-transferase were all increased by DR. Catalase activity was unaffected by DR but was increased by exercise. The following results demonstrate that long-term DR and exercise modulate the extent of free radical damage in the heart and enhance the antioxidant defense system.
自由基代谢可通过多种干预措施改变，包括饮食限制（DR）和运动。此前的大多数研究都集中在肝脏和骨骼肌上。进行以下实验以确定长期饮食限制和慢性运动是否会影响自由基代谢并改变心脏抗氧化防御状态。大鼠接受饮食限制和/或耐力运动18.5个月，然后与自由进食且 sedentary 的对照组一起处死。饮食限制和运动均降低了心脏线粒体丙二醛含量，表明脂质过氧化损伤减少。饮食限制使细胞溶质中的抗氧化酶超氧化物歧化酶、硒依赖性谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶均增加。过氧化氢酶活性不受饮食限制影响，但运动使其增加。以下结果表明，长期饮食限制和运动可调节心脏自由基损伤程度并增强抗氧化防御系统。
