Xu H P, Yanak B L, Wigler M H, Gorin M B
Cold Spring Harbor Laboratory, New York, USA.
Mamm Genome. 1996 Jan;7(1):16-9. doi: 10.1007/s003359900005.
We have used a Mus domesticus/-Mus spretus congenic animal that was selected for retention of Mus spretus DNA around the pearl locus to create a highly polymorphic region suitable for screening new markers. Representation difference analysis (RDA) was performed with either DNA from the congenic animal or C57BL/6J as the driver for subtraction. Four clones were identified, characterized, and converted to PCR-based polymorphic markers. Three of the four markers equally subdivide a 10-cM interval containing the pearl locus, with the fourth located centromeric to it. These markers have been placed on the mouse genetic map by use of an interspecific backcross panel between Mus domesticus (C57BL/6J) and Mus spretus generated by The Jackson Laboratory.
我们使用了一种小家鼠/西班牙小鼠同源动物，该动物被选择用于保留珍珠基因座周围的西班牙小鼠DNA，以创建一个适合筛选新标记的高度多态性区域。以同源动物或C57BL/6J的DNA作为驱动进行消减杂交的代表性差异分析（RDA）。鉴定、表征了四个克隆，并将其转化为基于PCR的多态性标记。四个标记中的三个将包含珍珠基因座的10厘摩区间平均细分，第四个位于其着丝粒侧。通过使用杰克逊实验室构建的小家鼠（C57BL/6J）和西班牙小鼠之间的种间回交群体，已将这些标记定位到小鼠遗传图谱上。
