Massele A Y, Nshimo C M
Department of Clinical Pharmacology, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania.
East Afr Med J. 1995 Oct;72(10):661-3.
A method utilizing Brine Shrimp (Artemia salina Leach) has been used to screen medicinal plants for biological activity. A number of twenty eight medicinal plants were collected from eastern part of Tanzania and their ethnobotanical uses obtained from traditional healers. Biological activity using the Brine Shrimp bioassay was recorded as a lethal concentration (LC 50) that kills 50% of the larvae within 24 hours of contact with the methanol plant extract. Of the 28 plant extracts tested eight gave an LC 50 of activity of less than 20 mu g/ml when compared with controls treated with 10% dimethylsulphoxide (DMS0) in water as solvent. Uvaria acuminata (Annonaceae) root extract was found to be the most active. The Brine Shrimp bioassay was found to be reliable, inexpensive and a convenient method for assessment of biologically active medicinal plants.
一种利用卤虫（卤虫属）的方法已被用于筛选具有生物活性的药用植物。从坦桑尼亚东部收集了28种药用植物，并从传统治疗师那里获得了它们的民族植物学用途。使用卤虫生物测定法记录的生物活性为致死浓度（LC50），即在与甲醇植物提取物接触24小时内杀死50%幼虫的浓度。与以10%二甲基亚砜（DMSO）为溶剂在水中处理的对照相比，在测试的28种植物提取物中，有8种的活性LC50小于20μg/ml。尖叶紫玉盘（番荔枝科）根提取物被发现活性最强。卤虫生物测定法被发现是一种可靠、廉价且方便的评估具有生物活性的药用植物的方法。
