Thurlow P J, Doolan L, Sharp R, Sullivan M, Smith B, Andersen L W
Departments of Haematology and Anaesthesia, Austin Hospital, Heidelberg, Australia.
Perfusion. 1996 Jan;11(1):29-37. doi: 10.1177/026765919601100104.
A total of 14 patients with ischaemic heart disease undergoing coronary artery bypass grafts were studied for the effect of AV6 control filter and LG6 neutrophil filter, used in the extracorporeal circulation, on different laboratory parameters. There was no statistical difference between the effects of AV6 and LG6 filters on total white cells, neutrophils, monocytes, lymphocytes, platelets or haemoglobin. The expression of neutrophil activation antigens identified with a panel of monoclonal antibodies demonstrated that for the LG6 filter the leucocyte tyrosine phosphate CD45Ro fell during the procedure, whilst there were no significant changes in any of the other neutrophil antigens. The AV6 filter did not significantly diminish the expression of any of the neutrophil antigens. An indirect measure of superoxide production using Dihydrorhodamine 123 identified that the more activated cells appeared to be depleted across the LG6 filter which was not evident with the AV6 filter. These studies indicate that the LG6 is not capable of significantly depleting the neutrophil load generated during extracorporeal circulation but may be capable of selectively removing the more activated forms.
对14例接受冠状动脉搭桥术的缺血性心脏病患者进行了研究，以探讨体外循环中使用的AV6控制过滤器和LG6中性粒细胞过滤器对不同实验室参数的影响。AV6过滤器和LG6过滤器对白细胞总数、中性粒细胞、单核细胞、淋巴细胞、血小板或血红蛋白的影响无统计学差异。用一组单克隆抗体鉴定的中性粒细胞活化抗原的表达表明，对于LG6过滤器，白细胞酪氨酸磷酸化CD45Ro在手术过程中下降，而其他任何中性粒细胞抗原均无显著变化。AV6过滤器并未显著降低任何中性粒细胞抗原的表达。使用二氢罗丹明123对超氧化物产生进行的间接测量表明，LG6过滤器能使更多活化细胞减少，而AV6过滤器则未出现此现象。这些研究表明，LG6不能显著减少体外循环期间产生的中性粒细胞负荷，但可能能够选择性地去除更多活化形式的中性粒细胞。
