Thurlow P J, Doolan L, Sharp R, Sullivan M, Smith B, Andersen L W
Department of Haematology, Austin Hospital, Heidelberg, Victoria, Australia.
Perfusion. 1995 Sep;10(5):291-300. doi: 10.1177/026765919501000503.
Neutrophil activation is thought to play a major role in the inflammatory response seen in reperfusion injury and similar clinical situations, i.e. extracorporeal circulation. Impairment of neutrophil function or reduction of total numbers of neutrophils using a leucocyte filter may be beneficial in reducing the adverse clinical effects. In this study we have investigated the effect of the Pall LG6 and control AV6 filters during simulated in vitro cardiopulmonary bypass (CPB). Various parameters were evaluated including neutrophils, total leucocytes, monocytes, lymphocytes and platelets, expression of antigens on neutrophils using a panel of leucocyte-associated monoclonal antibodies CD13, 14, 15, 45Ro, 67, 11a, 11b and L selectin. The effects of leucocyte stimulation with phorbol myristate acetate (PMA) and a leucocyte bolus from a patient with chronic myeloid leukaemia (CML) were also investigated. We have demonstrated that the LG6 significantly reduces leucocytes, in particular neutrophils, with a modest reduction of lymphocytes, platelets and haematocrit, whereas the AV6 had no effect on leukocytes or neutrophils in the test system. In addition the LG6 was associated with a reduction in expression of all leucocyte antigens by approximately 20%; however there was no appreciable alteration of any of the antigens with AV6. Leucocyte stimulation with PMA resulted in a dramatic decrease of all cellular elements and an extra leucocyte load (using CML leucocytes) was not effectively filtered by the LG6 filter. These studies identify the capacity of the LG6 as compared with the AV6 to deplete activated neutrophils in an in vitro simulated cardiopulmonary bypass circuit.
中性粒细胞活化被认为在再灌注损伤及类似临床情况（即体外循环）中所见的炎症反应中起主要作用。使用白细胞滤器损害中性粒细胞功能或减少中性粒细胞总数可能有助于减轻不良临床影响。在本研究中，我们研究了颇尔LG6过滤器和对照AV6过滤器在模拟体外心肺转流（CPB）期间的效果。评估了各种参数，包括中性粒细胞、总白细胞、单核细胞、淋巴细胞和血小板，使用一组白细胞相关单克隆抗体CD13、14、15、45Ro、67、11a、11b和L选择素检测中性粒细胞上抗原的表达。还研究了用佛波酯（PMA）刺激白细胞以及来自慢性粒细胞白血病（CML）患者的白细胞推注的效果。我们已经证明，LG6显著减少白细胞，特别是中性粒细胞，淋巴细胞、血小板和血细胞比容略有降低，而AV6在测试系统中对白细胞或中性粒细胞没有影响。此外，LG6与所有白细胞抗原的表达减少约20%相关；然而，AV6对任何抗原均无明显改变。用PMA刺激白细胞导致所有细胞成分急剧减少，并且额外的白细胞负荷（使用CML白细胞）未被LG6过滤器有效过滤。这些研究确定了与AV6相比，LG6在体外模拟心肺转流回路中消耗活化中性粒细胞的能力。
