将核糖体分型作为大肠杆菌O157血清型分离株分型的流行病学工具的评估。
Evaluation of ribotyping as epidemiologic tool for typing Escherichia coli serogroup O157 isolates.
作者信息
Martin I E, Tyler S D, Tyler K D, Khakhria R, Johnson W M
机构信息
Bureau of Microbiology, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
出版信息
J Clin Microbiol. 1996 Mar;34(3):720-3. doi: 10.1128/jcm.34.3.720-723.1996.
Abstract
A total of 121 representative Escherichia coli O157:H7 and O157:NM (nonmotile) isolates were characterized by ribotype, phage type, verotoxin genotype, and genomic fingerprints generated by pulsed-field gel electrophoresis. Ribotyping was not able to discriminate between O157:H7 isolates, and phage typing and pulsed-field gel electrophoresis were the most valuable and discriminatory techniques.
摘要
总共121株具有代表性的大肠杆菌O157:H7和O157:NM（无动力）分离株通过核糖体分型、噬菌体分型、志贺毒素基因型以及脉冲场凝胶电泳产生的基因组指纹图谱进行了特征分析。核糖体分型无法区分O157:H7分离株，而噬菌体分型和脉冲场凝胶电泳是最有价值且具有鉴别力的技术。
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