用于诊断牛疱疹病毒1型感染的免疫PCR技术的开发。
Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection.
作者信息
Mweene A S, Ito T, Okazaki K, Ono E, Shimizu Y, Kida H
机构信息
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Japan.
出版信息
J Clin Microbiol. 1996 Mar;34(3):748-50. doi: 10.1128/jcm.34.3.748-750.1996.
Abstract
A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 10(5.0) times higher than that of ELISA for antibody detection.
摘要
建立了一种高度灵敏的免疫聚合酶链反应方法，用于特异性检测牛疱疹病毒1的抗原和抗体。该检测方法具有很高的灵敏度，对于抗原检测，其灵敏度比酶联免疫吸附测定（ELISA）或聚合酶链反应（PCR）高10（7.0）倍；对于抗体检测，其灵敏度比ELISA高10（5.0）倍。
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