Cser M A, Sziklai-László I, Menzel H, Lombeck I
Bethesda Children's Hospital, Budapest, Hungary.
J Trace Elem Med Biol. 1996 Sep;10(3):167-73. doi: 10.1016/S0946-672X(96)80028-9.
Selenium (Se) in plasma, whole blood and erythrocytes as well as glutathione peroxidase (GSH-Px) activity in plasma and erythrocytes were investigated in 161 healthy Hungarian children aged 1-15 years. Se was determined by AAS with hydride generation. The estimation of GSH-Px activity was performed in plasma with tertbutyl-hydroperoxide (t-BOOH) and in erythrocytes with hydrogen peroxide (H2O2) as acceptor substrates. The Se content in plasma (0.63 +/- 0.12 mumol/L), whole blood (0.81 +/- 0.14 mumol/L) and erythrocytes (1.14 +/- 0.26 mumol/L, the GSH-Px activity in plasma (87 +/- 19 U/L) and erythrocytes (5.93 +/- 1.04 U/gHb) was low in Hungarian children in comparison to values for children from other European countries. Samples from a rural area in southeast Hungary showed even lower Se content than samples from an industrial city in the northwest or from the capital. The Se in plasma and whole blood as well as GSH-Px activity in the plasma exhibited a clear age dependency. There was a good correlation between plasma Se and GSH-Px activity in all children (r = 0.633, p < 0.001). In addition, in children from the northwestern city and from the capital a correlation was found between Se content and GSH-Px activity of erythrocytes (r = 0.625, p < 0.001). There is no indication that the high mortality in young Hungarian adults from cardiovascular diseases is mainly caused by a low Se supply because there are no corresponding findings in the surrounding countries of southeastern or central eastern Europe with similar low Se states.
对161名年龄在1至15岁的匈牙利健康儿童的血浆、全血和红细胞中的硒（Se）以及血浆和红细胞中的谷胱甘肽过氧化物酶（GSH-Px）活性进行了研究。采用氢化物发生原子吸收光谱法测定硒。以叔丁基过氧化氢（t-BOOH）为底物测定血浆中GSH-Px活性，以过氧化氢（H2O2）为底物测定红细胞中GSH-Px活性。与其他欧洲国家儿童的值相比，匈牙利儿童血浆中硒含量（0.63±0.12μmol/L）、全血中硒含量（0.81±0.14μmol/L）和红细胞中硒含量（1.14±0.26μmol/L），以及血浆中GSH-Px活性（87±19U/L）和红细胞中GSH-Px活性（5.93±1.04U/gHb）较低。匈牙利东南部农村地区的样本硒含量甚至低于西北部工业城市或首都的样本。血浆和全血中的硒以及血浆中的GSH-Px活性呈现出明显的年龄依赖性。所有儿童血浆硒与GSH-Px活性之间存在良好的相关性（r = 0.633，p < 0.001）。此外，在西北部城市和首都的儿童中，发现红细胞中硒含量与GSH-Px活性之间存在相关性（r = 0.625，p < 0.001）。没有迹象表明匈牙利年轻成年人中心血管疾病的高死亡率主要是由低硒供应引起 的，因为在东南欧或中东欧周边硒水平相似的国家没有相应的发现。
