Abe M, Suzuki O, Tasaki K, Abe R, Wakasa H
Department of Pathology, Fukushima Medical College, Japan.
Pathol Int. 1996 Sep;46(9):630-8. doi: 10.1111/j.1440-1827.1996.tb03665.x.
Two newly established human Burkitt's lymphoma cell lines (HBL-7 and HBL-8) were characterized by immunophenotypic, cytogenetic and molecular studies. Both cell lines were negative for Epstein-Barr virus (EBV) genome and had chromosomal translocation: t(8;14) (q24;q32). Immunoglobulin (Ig) gene rearrangement analyses confirmed that both cell lines were derived from primary lymphoma cells. These cell lines were heterotransplanted subcutaneously into severe combined immunodeficiency (SCID) mice to investigate the metastatic capacity. The most striking feature of both cell lines was to show highly spontaneous metastasis to distant organs, particularly spleen, bone marrow and ovaries in SCID mice. To elucidate the metastatic factors involved in the process of spontaneous metastasis, cell surface adhesion molecules or extracellular matrix receptors were analyzed. However, the results did not allow a significant correlation between expression levels of those molecules or matrix receptors and spontaneous metastasis in the SCID mouse model. The HBL-7 and HBL-8 cell lines, however, may be a useful tool to elucidate the metastatic mechanisms of human lymphomas in an animal model.
通过免疫表型、细胞遗传学和分子研究对两个新建立的人类伯基特淋巴瘤细胞系（HBL - 7和HBL - 8）进行了特征分析。这两个细胞系的爱泼斯坦 - 巴尔病毒（EBV）基因组均为阴性，且都有染色体易位：t(8;14)(q24;q32)。免疫球蛋白（Ig）基因重排分析证实这两个细胞系均源自原发性淋巴瘤细胞。将这些细胞系皮下异种移植到严重联合免疫缺陷（SCID）小鼠体内以研究其转移能力。这两个细胞系最显著的特征是在SCID小鼠中表现出高度自发转移至远处器官，尤其是脾脏、骨髓和卵巢。为了阐明自发转移过程中涉及的转移因子，对细胞表面黏附分子或细胞外基质受体进行了分析。然而，结果并未显示这些分子或基质受体的表达水平与SCID小鼠模型中的自发转移之间存在显著相关性。不过，HBL - 7和HBL - 8细胞系可能是在动物模型中阐明人类淋巴瘤转移机制的有用工具。
