Abe M, Nozawa Y, Wakasa H, Ohno H, Fukuhara S
Department of Pathology, Fukushima Medical College, Japan.
Cancer. 1988 Feb 1;61(3):483-90. doi: 10.1002/1097-0142(19880201)61:3<483::aid-cncr2820610313>3.0.co;2-l.
Two Epstein-Barr virus (EBV)-negative lymphoma B-cell lines, HBL-1 and HBL-2, were established from a pleural effusion and a lymph node biopsy of two patients with diffuse large cell lymphoma. HBL-1 and HBL-2 showed the characteristics of activated B-cells in B-cell lineage, as did original lymphoma cells. Chromosome analyses revealed that HBL-1 exhibiting 14q+ marker-positive lymphoid cancer showed a new subclass of 14q32 translocation resulting from a translocation between chromosomes 14 and 16, which had been masked in a complex translocation involving five chromosomes, and that HBL-2 had a 14q+ marker chromosome, the result of an 11;14 translocation [t(11;14)(q13;32)]. Successful heterotransplantation into athymic nude mice demonstrated tumorigenicity of HBL-1 and HBL-2. The transplantability and tumor growth rate of HBL-2 were higher and more rapid than those of HBL-1. HBL-1 and HBL-2 appear useful for facilitating therapeutic investigations as well as immunologic and oncogenic studies in B-cell lymphomas.
从两名弥漫性大细胞淋巴瘤患者的胸腔积液和淋巴结活检中建立了两株爱泼斯坦-巴尔病毒（EBV）阴性淋巴瘤B细胞系，即HBL-1和HBL-2。HBL-1和HBL-2表现出B细胞谱系中活化B细胞的特征，原始淋巴瘤细胞也是如此。染色体分析显示，表现出14q+标记阳性的淋巴癌的HBL-1显示出一种新的14q32易位亚类，该易位是由14号和16号染色体之间的易位导致的，在涉及五条染色体的复杂易位中被掩盖，而HBL-2有一条14q+标记染色体，是11；14易位[t(11;14)(q13;32)]的结果。成功地将其异种移植到无胸腺裸鼠体内证明了HBL-1和HBL-2的致瘤性。HBL-2的移植能力和肿瘤生长速度比HBL-1更高、更快。HBL-1和HBL-2似乎有助于促进B细胞淋巴瘤的治疗研究以及免疫和致癌研究。
