Joshi S S, DeBoer J M, Strandjord S J, Pirruccello S J, Sanger W G, Weisenburger D D, Sharp J G
Department of Anatomy, University of Nebraska Medical Center, Omaha 68198-6395.
Int J Cancer. 1991 Mar 12;47(5):643-8. doi: 10.1002/ijc.2910470502.
Using culture techniques, we have been able to grow occult tumor cells from the bone marrow from cancer patients and have developed a new malignant lymphoid cell line, OMA-BL-1, from the bone marrow of a 17-year-old patient with recurrent Burkitt's lymphoma. The tumor cells grew rapidly in vitro in suspension culture, and very aggressively in vivo in athymic nude mice with metastases to the liver and abdominal cavity. The morphological, chromosomal, immunophenotypic and molecular biologic characteristics of fresh uncultured tumor cells from the patient and tumor cells grown in culture and in athymic nude mice were very similar. The cells were positive for Epstein-Barr virus-associated nuclear antigens (EBNA) and chromosome analysis of the cells revealed an atypical chromosomal abnormality of 45,X,-X,i(8q), HSR(18)(q21),t(8;14)(q24;q32). Southern analysis demonstrated that c-myc was rearranged and amplified in these cells. Immunophenotypic analysis of the cells using flow cytometry showed monoclonal B cells expressing a surface IgG-kappa isotype. The tumor cells grown in nude mice had a significant decrease in CD24 expression when compared to cultured tumor cells. Electron microscopy of the fresh and cultured cells revealed Herpes virus, most likely Epstein-Barr virus, particles. This cell line has been maintained in culture for over 18 months. The aggressive growth and metastatic properties of this cell line in athymic nude mice make it a potentially useful experimental model to study the biology of human lymphoma.
通过培养技术，我们已能够从癌症患者的骨髓中培养出隐匿性肿瘤细胞，并从一名患有复发性伯基特淋巴瘤的17岁患者的骨髓中建立了一种新的恶性淋巴样细胞系OMA-BL-1。肿瘤细胞在体外悬浮培养中生长迅速，在无胸腺裸鼠体内生长极为活跃，并转移至肝脏和腹腔。患者新鲜未培养的肿瘤细胞以及在培养物和无胸腺裸鼠体内生长的肿瘤细胞的形态学、染色体、免疫表型和分子生物学特征非常相似。这些细胞对爱泼斯坦-巴尔病毒相关核抗原（EBNA）呈阳性，细胞染色体分析显示存在45,X,-X,i(8q), HSR(18)(q21),t(8;14)(q24;q32)的非典型染色体异常。Southern分析表明，这些细胞中的c-myc发生了重排和扩增。使用流式细胞术对细胞进行免疫表型分析显示，单克隆B细胞表达表面IgG-κ同种型。与培养的肿瘤细胞相比，在裸鼠体内生长的肿瘤细胞的CD24表达显著降低。新鲜细胞和培养细胞的电子显微镜检查发现了疱疹病毒颗粒，很可能是爱泼斯坦-巴尔病毒颗粒。该细胞系已在培养中维持了18个多月。该细胞系在无胸腺裸鼠体内的侵袭性生长和转移特性使其成为研究人类淋巴瘤生物学的潜在有用实验模型。
