Sakamoto S, Oki K, Takahashi H, Arakawa Y, Sugita H, Hirano H, Takeuchi K, Tomichi N, Sakuma T, Futai K, Kawabata Y
Department of Respiratory Medicine, Iwate Prefectural Central Hospital, Morioka, Japan.
Int Arch Allergy Immunol. 1996;111 Suppl 1:26-8. doi: 10.1159/000237410.
Recent studies have suggested that there may be heterogeneity among human eosinophils. To study this further, surface antigens on blood eosinophils from patients with eosinophilia (23 bronchial asthma, 6 eosinophilic pneumonia, 1 Kimura's disease and 1 adult T-cell leukemia) and from 8 control subjects were examined using a new direct method for fluorescence detection of eosinophils. HLA-DR+ and CD4+ eosinophil counts were higher in patients with bronchial asthma and adult T-cell leukemia (ATL) than in patients from other groups and in control subjects. CD11b+ eosinophil counts in Kimura's disease and ATL were smaller than those in the other groups. CD45RO+ eosinophil counts in bronchial asthma and eosinophilic pneumonia were significantly higher (p < 0.05) compared with Kimura's disease, ATL and control subjects. CD44+ eosinophil counts in eosinophilic pneumonia were significantly higher (p < 0.05) compared with the other groups and control subjects. These results suggest the existence of functional heterogeneity in the different eosinophilic diseases, with eosinophils in bronchial asthma and eosinophilic pneumonia being more highly activated in migration, activation and immunoregulation. On the other hand, eosinophils in Kimura's disease and ATL might be functionally down-regulated. This heterogeneity of eosinophils may reflect differences in the pathogenesis of various eosinophilic diseases.
近期研究表明，人类嗜酸性粒细胞之间可能存在异质性。为进一步研究这一问题，采用一种新的嗜酸性粒细胞荧光检测直接方法，对嗜酸性粒细胞增多症患者（23例支气管哮喘、6例嗜酸性粒细胞性肺炎、1例木村病和1例成人T细胞白血病）及8名对照者血液中的嗜酸性粒细胞表面抗原进行了检测。支气管哮喘患者和成人T细胞白血病患者的HLA-DR+和CD4+嗜酸性粒细胞计数高于其他组患者及对照者。木村病和成人T细胞白血病患者的CD11b+嗜酸性粒细胞计数低于其他组。与木村病、成人T细胞白血病患者及对照者相比，支气管哮喘和嗜酸性粒细胞性肺炎患者的CD45RO+嗜酸性粒细胞计数显著更高（p < 0.05）。与其他组及对照者相比，嗜酸性粒细胞性肺炎患者的CD44+嗜酸性粒细胞计数显著更高（p < 0.05）。这些结果提示，不同嗜酸性粒细胞疾病中存在功能异质性，支气管哮喘和嗜酸性粒细胞性肺炎中的嗜酸性粒细胞在迁移、激活和免疫调节方面的激活程度更高。另一方面，木村病和成人T细胞白血病中的嗜酸性粒细胞功能可能下调。嗜酸性粒细胞的这种异质性可能反映了各种嗜酸性粒细胞疾病发病机制的差异。
