Takahashi N, Maeda R, Shibuya T
Department of Parasitology, Teikyo University School of Medicine, Tokyo, Japan.
Int Arch Allergy Immunol. 1996;111 Suppl 1:51-4. doi: 10.1159/000237416.
In order to study the role of eosinophils in filarial infection, ddY mice were inoculated with 20 Litomosoides carinii 7-day larvae each by the artificial pneumothorax technique. Migrated cells and larvae were collected from the thoracic cavity of the infected mice weekly from the 1st to the 8th week after infection and prepared for transmission electron microscopic observation. The number of migrated eosinophils and the proportion of eosinophils in the total migrated cells reached a peak at the 5th week. At the same time, eosinophils with low density granules were observed in both unattached and attached cells. In particular, many attached eosinophils with low density granules were observed directly on the worm surface. These observations strongly suggest that eosinophils are associated with attack on filarial worms.
为了研究嗜酸性粒细胞在丝虫感染中的作用，采用人工气胸技术给ddY小鼠接种20条卡氏罗阿丝虫7日龄幼虫。在感染后的第1周至第8周，每周从感染小鼠的胸腔收集游走细胞和幼虫，并制备用于透射电子显微镜观察。游走嗜酸性粒细胞的数量和嗜酸性粒细胞在总游走细胞中的比例在第5周达到峰值。同时，在未附着和附着的细胞中均观察到低密度颗粒的嗜酸性粒细胞。特别是，在虫体表面直接观察到许多带有低密度颗粒的附着嗜酸性粒细胞。这些观察结果有力地表明，嗜酸性粒细胞与对丝虫的攻击有关。
