da Silva N S, Dias Filho B P, De Souza W
Laboratório de Biologia Celular e Tecidual, Biotecnologia-Universidade Estadual do Norte Fluminense-UENF, Rio de Janeiro, Brasil.
Cell Struct Funct. 1996 Aug;21(4):245-50. doi: 10.1247/csf.21.245.
Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetus-erythrocytes attachment showed that trichomonad cytoadherence took place mainly through their anterior and recurrent flagella. Ultrastructural observations showed that T. foetus contacts human red blood cells through punctual binding, inducing the separation between the two lipid monolayers of the parasite plasma membrane. This structural modification was also seen in freeze-fracture replicas where protrusions on the P and depressions on the E fracture faces were observed. No intramembranous particles, which mainly correspond to membrane integral proteins, were observed at the adhesion areas, indicating lateral mobility of integral membrane components involved in the appearance of the intramembranous particles. However, no changes was observed on the surface coat.
胎儿三毛滴虫能强烈凝集人红细胞，因此提示其表面存在一种粘附素。将寄生虫与红细胞混合后立即观察到粘附现象，且强度在30分钟内不断增加。对胎儿三毛滴虫与红细胞附着的扫描电子显微镜检查显示，滴虫细胞粘附主要通过其前鞭毛和复鞭毛进行。超微结构观察表明，胎儿三毛滴虫通过点状结合与人红细胞接触，导致寄生虫质膜的两个脂质单层之间分离。在冷冻蚀刻复制品中也观察到这种结构改变，其中在P面观察到突起，在E面观察到凹陷。在粘附区域未观察到主要对应于膜整合蛋白的膜内颗粒，表明参与膜内颗粒出现的整合膜成分具有侧向流动性。然而，表面涂层未观察到变化。
