使用白光共聚焦显微镜诊断细菌性隐形眼镜相关性角膜炎。
Diagnosis of bacterial contact lens related keratitis with the white-light confocal microscope.
作者信息
Kaufman S C, Laird J A, Cooper R, Beuerman R W
机构信息
Lions Eye Research Laboratories, LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112, USA.
出版信息
CLAO J. 1996 Oct;22(4):274-7.
PURPOSE
We used white-light confocal microscopy to identify the causative organisms in two patients with contact lens related keratitis.
METHODS
The corneal infiltrates were examined by confocal microscopy and corneal specimens were obtained for culture.
RESULTS
In both patients, confocal microscopy revealed 1.5- to 2-micron diameter hyper-reflective bodies below the level of the epithelium. The sizes of these structures were consistent with those of bacteria. The corneal cultures from one patient grew Staphylococcus werneri and the cultures from the other patient grew Streptococcus viridans.
CONCLUSIONS
The confocal microscope permitted the in vivo observation of what we believe to be bacteria in patients diagnosed with contact lens related keratitis.
目的
我们使用白光共聚焦显微镜来鉴定两名与隐形眼镜相关的角膜炎患者的致病微生物。
方法
通过共聚焦显微镜检查角膜浸润，并获取角膜标本进行培养。
结果
在两名患者中，共聚焦显微镜均显示上皮层以下有直径为1.5至2微米的高反射体。这些结构的大小与细菌的大小一致。一名患者的角膜培养物培养出维内氏葡萄球菌，另一名患者的培养物培养出绿色链球菌。
结论
共聚焦显微镜能够对被诊断为与隐形眼镜相关的角膜炎患者体内我们认为是细菌的物质进行活体观察。
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