Marson L, McKenna K E
Division of Urology, University of North Carolina School of Medicine, Chapel Hill 27599, USA.
J Comp Neurol. 1996 Oct 14;374(2):161-79. doi: 10.1002/(SICI)1096-9861(19961014)374:2<161::AID-CNE1>3.0.CO;2-0.
Transneuronal tracing techniques were used to identify spinal and brainstem neurons involved in the control of perineal muscles in the male rat. Two penile muscles, the bulbospongiosus and ischiocavernosus muscles, were injected with Bartha's strain of pseudorabies virus. After survival periods of 2, 4, and 5 days, the rats were killed and viral labeled neurons identified by immunohistochemistry. After a 2 day survival period, only pudendal motoneurons were labeled. More spinal and brainstem neurons were labeled at longer survival times. Putative spinal interneurons were found from T13 to S1. Large numbers of neurons were found in the lateral horn of the T13-L2 and L6-S1 segments which contain sympathetic and parasympathetic preganglionic neurons, respectively. However, retrograde labeling experiments verified that very few of the viral neurons were preganglionic neurons. Other labeled neurons were found in the intermediate cord, especially around the central canal. Relatively few labeled neurons were seen in the dorsal or ventral horn. In the brainstem, consistent labeling was seen in the ventrolateral medulla, raphe pallidus, and magnus, the A5 and locus ceruleus noradrenergic cell groups. Barrington's nucleus in the pontine tegmentum, the periaqueductal gray, and the paraventricular nucleus of the hypothalamus. The transneuronal labeling was consistent with what is currently known of the central nervous system (CNS) control of the perineal muscles.
运用跨神经元追踪技术来识别参与雄性大鼠会阴肌控制的脊髓和脑干神经元。向球海绵体肌和坐骨海绵体肌这两块阴茎肌注射巴塔株伪狂犬病病毒。在存活2天、4天和5天后，处死大鼠，并通过免疫组织化学鉴定病毒标记的神经元。在2天的存活期后，仅阴部运动神经元被标记。在更长的存活时间有更多的脊髓和脑干神经元被标记。在T13至S1发现了假定的脊髓中间神经元。在T13 - L2节段和L6 - S1节段的外侧角发现了大量神经元，它们分别包含交感和副交感神经节前神经元。然而，逆行标记实验证实，病毒标记的神经元中很少是节前神经元。在脊髓中间带也发现了其他标记神经元，尤其是围绕中央管的区域。在背角或腹角可见相对较少的标记神经元。在脑干中，在腹外侧延髓、中缝苍白核、中缝大核、A5和蓝斑去甲肾上腺素能细胞群可见一致的标记。脑桥被盖中的巴林顿核、导水管周围灰质和下丘脑室旁核也有标记。跨神经元标记与目前已知的中枢神经系统（CNS）对会阴肌的控制情况一致。
