Weber J D, Isakson P C, Purkerson J M
Monsanto Company/Searle Research and Development, Immunoinflammatory Diseases Department, St. Louis, MO 63198, USA.
J Immunol. 1996 Nov 15;157(10):4428-35.
Interleukin-5 plays a pivotal role in the regulation of Ig secretion from murine B cells. Resting B cells express few, if any, IL-5 receptors and do not respond to the lymphokine. Culture of resting B cells with IL-4 induced expression of the IL-5R alpha-chain, while signaling through membrane Ig stimulated expression of the IL-5R beta-chain. Surprisingly, IL-4 suppressed expression of the beta-chain on activated B cells and inhibited responsiveness to IL-5 in subsequent cultures. Simultaneous culture of B lymphoblasts with IL-4 and IL-5 elicited sustained expression of the beta-chain and promoted secretion of IgM. Thus, expression of IL-5 receptors and induction of Ig secretion requires coordination of signals provided by membrane Ig, IL-4, and IL-5.
白细胞介素-5在调节小鼠B细胞免疫球蛋白分泌中起关键作用。静止B细胞即使有也很少表达白细胞介素-5受体，并且对该淋巴因子无反应。用白细胞介素-4培养静止B细胞可诱导白细胞介素-5Rα链的表达，而通过膜免疫球蛋白发出的信号则刺激白细胞介素-5Rβ链的表达。令人惊讶的是，白细胞介素-4抑制活化B细胞上β链的表达，并在随后的培养中抑制对白细胞介素-5的反应性。B淋巴母细胞与白细胞介素-4和白细胞介素-5同时培养可引发β链的持续表达并促进IgM的分泌。因此，白细胞介素-5受体的表达和免疫球蛋白分泌的诱导需要膜免疫球蛋白、白细胞介素-4和白细胞介素-5提供的信号协调。
