Vissers P M, DeGrip W J
Institute of Cellular Signalling, Department of Biochemistry, University of Nijmegen, The Netherlands.
FEBS Lett. 1996 Oct 28;396(1):26-30. doi: 10.1016/0014-5793(96)01064-2.
Mammalian color vision is mediated by light-sensitive pigments in retinal cone cells. Biochemical studies on native mammalian cone visual pigments are seriously hampered by their low levels and instability. We describe a novel approach for their functional expression, employing the baculovirus system in combination with histidine tagging to allow future purification and structural analysis. The human red and green cone pigments are produced in relatively large amounts and can be detected by immunocytochemistry as well as by immunoblotting. Histidine tagging has no significant effect on the absorbance maxima. The first evidence is presented that these pigments are N-glycosylated.
哺乳动物的色觉由视网膜锥细胞中的光敏色素介导。对天然哺乳动物锥视觉色素的生化研究因它们的低水平和不稳定性而受到严重阻碍。我们描述了一种用于其功能表达的新方法，该方法采用杆状病毒系统并结合组氨酸标签，以便未来进行纯化和结构分析。人红色和绿色锥色素能大量产生，并且可以通过免疫细胞化学以及免疫印迹法检测到。组氨酸标签对最大吸光度没有显著影响。首次有证据表明这些色素是N-糖基化的。
