Holness M J
Department of Biochemistry, Medical Sciences, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, UK.
FEBS Lett. 1996 Oct 28;396(1):53-6. doi: 10.1016/0014-5793(96)01067-8.
Low (8%) protein feeding during pregnancy impairs the functional development of the fetal endocrine pancreas. Continued low-protein feeding post-natally decreases pancreatic insulin content and secretion, whereas transfer to standard diet evoked beta-cell recuperation. Glucose-stimulated insulin secretion (GSIS) and insulin action were examined in vivo at 28 days after transfer from 8% protein diet to a high-energy/high-fat/standard (20%)-protein diet (HEF diet). HEF feeding dramatically enhanced GSIS after intravenous glucose in control rats, but not in rats previously maintained on the low-protein diet. However, glucose disappearance after intravenous glucose, and glucose production and whole-body glucose disposal during euglycaemic-hyperinsulinaemic clamps were unaffected by prior protein malnutrition. In conclusion, impaired insulin secretion after protein malnutrition is exacerbated by high-energy/high-fat feeding, but this response is not linked to enhanced whole-body insulin resistance.
孕期低蛋白（8%）喂养会损害胎儿内分泌胰腺的功能发育。出生后持续低蛋白喂养会降低胰腺胰岛素含量和分泌，而转为标准饮食可促使β细胞恢复。在从8%蛋白饮食转为高能量/高脂肪/标准（20%）蛋白饮食（HEF饮食）28天后，对体内葡萄糖刺激的胰岛素分泌（GSIS）和胰岛素作用进行了检测。HEF喂养显著增强了对照大鼠静脉注射葡萄糖后的GSIS，但对先前维持低蛋白饮食的大鼠则无此作用。然而，静脉注射葡萄糖后的葡萄糖消失，以及正常血糖-高胰岛素钳夹期间的葡萄糖生成和全身葡萄糖处置不受先前蛋白质营养不良的影响。总之，蛋白质营养不良后胰岛素分泌受损会因高能量/高脂肪喂养而加剧，但这种反应与全身胰岛素抵抗增强无关。
