Wojcik J
Department of Carnivore and Fur Animal Diseases, National Veterinary Research Institute, Pulawy, Poland.
Acta Microbiol Pol. 1995;44(2):191-6.
The selected part of gag gene (nucleotide sequences at position 618 to 1724) coding for P24 and P15 proteins of feline immunodeficiency virus (FIV) was expressed in vaccinia virus vector under control of 7.5 kDa vaccinia virus transtriptional signal (p 7.5 vaccinia virus early/late promoter). The vaccinia virus recombinant (denoted VV-G(1)) was generated by homologous recombination following CV-1 cells transfection with a newly prepared vaccinia virus insertion vector -- pWKr75.
猫免疫缺陷病毒（FIV）编码P24和P15蛋白的gag基因选定部分（第618至1724位核苷酸序列）在痘苗病毒转录信号（p7.5痘苗病毒早期/晚期启动子）控制下于痘苗病毒载体中表达。通过用新制备的痘苗病毒插入载体——pWKr75转染CV-1细胞后进行同源重组，产生了痘苗病毒重组体（命名为VV-G(1)）。
