Rankin C A, Ziemer D M, Maser R L, Foo I, Calvet J P
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160-7421, USA.
In Vitro Cell Dev Biol Anim. 1996 Feb;32(2):100-6. doi: 10.1007/BF02723041.
Polycystic kidney disease (PKD) is characterized by multiple renal cysts that are lined by epithelium and filled with fluid. PKD may result from one of a number of factors, either inherited or environmental. In this study, we have compared two mouse models in which PKD results from a genetic cause. In the C57BL/6J-cpk model, the mutated gene is unknown. In the other model, an SV40 large T antigen transgene causes renal cysts. We examined cultured cells from the kidneys of these mouse models, comparing growth characteristics. Although several features of PKD lead one to expect that the epithelial cells lining the cysts would have an increased rate of proliferation in culture, we found that they did not. The implications of these findings are discussed.
多囊肾病（PKD）的特征是多个肾囊肿，囊肿内衬上皮细胞并充满液体。PKD可能由多种因素之一引起，这些因素可以是遗传的或环境的。在本研究中，我们比较了两种因遗传原因导致PKD的小鼠模型。在C57BL/6J-cpk模型中，突变基因未知。在另一个模型中，SV40大T抗原转基因导致肾囊肿。我们检查了来自这些小鼠模型肾脏的培养细胞，比较其生长特性。尽管PKD的几个特征让人预期囊肿内衬的上皮细胞在培养中的增殖速率会增加，但我们发现并非如此。本文讨论了这些发现的意义。
