Kametani S, Mizuno H, Shiga Y, Akanuma H
Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku.
J Biochem. 1996 Jan;119(1):180-5. doi: 10.1093/oxfordjournals.jbchem.a021206.
Of the all-carbon-13 compounds, glucose is one of the most easily accessible, and therefore we applied 13C-NMR technique to the metabolic study of glucose-related compounds, 1,5-anhydro-D-glucitol and 1,5-anhydro-D-fructose (AF). Applying an INADEQUATE method to the substitutes of these novel sugars fully labeled with carbon-13, we could trace out the entire carbon skeleton with high sensitivity and confirm the chemical structures of these sugars. The method also provided a much easier way to optimize the enzymatic oxidation for AF preparation: we selectively and continuously monitored the quantities, as well as their structures in aqueous solution, of the substrate and products in a noninvasive manner. Similarly relying upon information from the 13C-NMR, we developed a valuable derivatization method of AF for its GC-MS application, which was so sensitive that we were able to demonstrate the natural occurrence of AF in rat liver.
在所有含碳-13的化合物中，葡萄糖是最容易获取的化合物之一，因此我们将碳-13核磁共振技术应用于与葡萄糖相关的化合物、1,5-脱水-D-葡萄糖醇和1,5-脱水-D-果糖（AF）的代谢研究。将异核单量子关联谱方法应用于这些完全用碳-13标记的新型糖类的替代物上，我们能够以高灵敏度追踪出整个碳骨架，并确认这些糖类的化学结构。该方法还提供了一种更简便的方式来优化用于制备AF的酶促氧化反应：我们以非侵入性方式选择性地、持续地监测水溶液中底物和产物的量及其结构。同样依靠来自碳-13核磁共振的信息，我们开发了一种用于AF气相色谱-质谱联用分析的有价值的衍生化方法，该方法灵敏度极高，以至于我们能够证明AF在大鼠肝脏中的天然存在。
