Navenot J M, Bernard D, Harousseau J L, Muller J Y, Blanchard D
Centre Regional de Transfusion Sanguine, Nantes, France.
Leuk Lymphoma. 1996 Mar;21(1-2):143-51. doi: 10.3109/10428199609067592.
We used CD55 and CD59 monoclonal antibodies (mAbs) for the investigation of peripheral blood cells from sixteen patients with paroxysmal nocturnal haemoglobinuria (PNH) by flow cytometry. Mixed-field populations of erythrocytes, platelets, monocytes or granulocytes were visualized in all cases but, due to the extended half-life of unaltered erythrocytes and blood transfusions, the GPI-linked protein deficiency was more obvious for white blood cells, especially granulocytes, and platelets. In contrast, a minority of altered lymphocytes was observed in only five patients. The expression of CD55 and CD59 antigens was grossly correlated but quantitative differences were observed for patients whose cells were only partially deficient in GPI-linked proteins. Patients with PNH secondary to aplastic anaemia exhibited a significantly lower percentage of altered cells compared with "classical" PNH patients. In addition, we found that CD58 normally bound to deficient white blood cells and platelets, showing the LFA-3 molecule is expressed as a transmembrane protein in these cells.
我们使用CD55和CD59单克隆抗体（mAbs），通过流式细胞术对16例阵发性夜间血红蛋白尿（PNH）患者的外周血细胞进行研究。在所有病例中均可见红细胞、血小板、单核细胞或粒细胞的混合视野群体，但由于未改变的红细胞和输血的半衰期延长，糖基磷脂酰肌醇（GPI）连接蛋白缺乏在白细胞尤其是粒细胞和血小板中更为明显。相比之下，仅在5例患者中观察到少数改变的淋巴细胞。CD55和CD59抗原的表达总体相关，但对于GPI连接蛋白仅部分缺乏的患者，观察到了定量差异。再生障碍性贫血继发的PNH患者与“经典”PNH患者相比，改变细胞的百分比显著更低。此外，我们发现CD58通常与缺陷的白细胞和血小板结合，表明淋巴细胞功能相关抗原-3（LFA-3）分子在这些细胞中作为跨膜蛋白表达。
