Smeets E F, Comfurius P, Bevers E M, Zwaal R F
Cardiovascular Research Institute Maastricht, Department of Biochemistry, University of Limburg, The Netherlands.
Thromb Res. 1996 Feb 15;81(4):419-26. doi: 10.1016/0049-3848(96)00014-x.
The influence of different neutral phospholipids and cholesterol on the procoagulant properties of sonicated vesicles containing phosphatidylserine was studied, using the prothrombinase assay. When incorporated into membranes composed of phosphatidylcholine and phosphatidylserine, a stimulating effect of phosphatidylethanolamine and an inhibiting effect of sphingomyelin was observed. Cholesterol slightly increased the activities of all vesicles tested. In lipid vesicles with a composition mimicking that of the outer leaflet of the plasma membrane of the activated platelet, the inhibitory effect of sphingomyelin was overruled by an overall stimulatory effect of phosphatidylethanolamine, suggesting an accessory role for phosphatidylethanolamine in the procoagulant properties of activated platelets.
采用凝血酶原酶测定法，研究了不同中性磷脂和胆固醇对含磷脂酰丝氨酸的超声处理囊泡促凝特性的影响。当将其掺入由磷脂酰胆碱和磷脂酰丝氨酸组成的膜中时，观察到磷脂酰乙醇胺具有刺激作用，而鞘磷脂具有抑制作用。胆固醇使所有测试囊泡的活性略有增加。在组成模拟活化血小板质膜外小叶的脂质囊泡中，鞘磷脂的抑制作用被磷脂酰乙醇胺的整体刺激作用所抵消，这表明磷脂酰乙醇胺在活化血小板的促凝特性中起辅助作用。
