Aoki N, Mori M, Kato K, Sakamoto Y, Noda K, Tajima M, Shimada H
Department of Hematology, Tokyo Metropolitan Geriatric Hospital, Japan.
Neurosci Lett. 1996 Feb 23;205(2):111-4. doi: 10.1016/0304-3940(96)12389-2.
Antibody against JC virus (JCV) was raised in rabbits with the use of synthetic multiple antigen peptides. The peptide sequences were derived from three regions of JCV VP1 protein, which showed less similarity with BK virus (BKV) counterpart. The antibodies raised with these peptides were designated as JCAb1, 2 and 3. JCAb1 specifically reacted with JCV and not with BKV, while JCAb2 and 3 reacted both with JCV and BKV. All of these antibodies reacted with JCV antigen of formalin-fixed paraffin sections of progressive multifocal leukoencephalopathy (PML) brain tissue. As JCAb1 is JCV-specific and reacted with JCV in formalin-fixed paraffin sections, it will contribute not only to rapid and accurate immunohistochemical diagnoses of PML but also to clarification of the pathogenesis of JCV infection.
利用合成多抗原肽在兔体内制备了抗JC病毒（JCV）抗体。肽序列来源于JCV VP1蛋白的三个区域，这些区域与BK病毒（BKV）的对应区域相似度较低。用这些肽制备的抗体分别命名为JCAb1、JCAb2和JCAb3。JCAb1与JCV特异性反应，不与BKV反应，而JCAb2和JCAb3与JCV和BKV均有反应。所有这些抗体都与进行性多灶性白质脑病（PML）脑组织福尔马林固定石蜡切片中的JCV抗原发生反应。由于JCAb1具有JCV特异性且能与福尔马林固定石蜡切片中的JCV发生反应，它不仅将有助于PML的快速准确免疫组化诊断，还将有助于阐明JCV感染的发病机制。
