Van Meirvenne N, Magnus E, Buscher P
Laboratory of Serology, Institute of Tropical Medicine, Antwerp, Belgium.
Acta Trop. 1995 Dec;60(3):189-99. doi: 10.1016/0001-706x(95)00127-z.
Twelve T.b. gambiense clone populations of distinct Variable Antigen Type (VAT) were combined in immune lysis tests with 340 sera of trypanosome infected patients from 8 different African countries and 267 non trypanosomiasis control sera. The diagnostic specificity of the test was 100%. At a serum dilution of 1:4 the overall test sensitivity with single VATs varied from 39.1 to 98.2% and from 12.1 to 86.8% at 1:32. At a serum dilution of 1:32 some combination tests with 2 VATs still scored above 96%. The VAT recognition patterns were clearly correlated with the geographical origin of the sera, reflecting a diversity in variable antigen repertoires.
将12个不同可变抗原类型（VAT）的布氏冈比亚锥虫克隆群体与来自8个不同非洲国家的340例锥虫感染患者的血清以及267例非锥虫病对照血清进行免疫裂解试验。该试验的诊断特异性为100%。在血清稀释度为1:4时，单个VAT的总体试验敏感性在39.1%至98.2%之间，在1:32时为12.1%至86.8%。在血清稀释度为1:32时，一些使用2个VAT的联合试验得分仍高于96%。VAT识别模式与血清的地理来源明显相关，反映了可变抗原库的多样性。
