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[利用聚合酶链反应检测儿童和青少年中的分枝杆菌]

[The detection of mycobacteria in children and adolescents by using the polymerase chain reaction].

作者信息

Golyshevskaia V I, Gintsburg A L, Levi D T, Mitinskaia L A, Korneev A A, Shaginian I A, Nesterenko L N, Grishina T D, Iukhimenko N V, Grigor'eva Z P

出版信息

Probl Tuberk. 1996(1):27-9.

PMID:8907481
Abstract

A polymerase chain reaction (PCR) was used for determination of mycobacterial DNA in clinical samples from children and adolescents. The results were positive in 23 and 53% of cases, respectively, while standard microbiological methods failed to show presence of any bacteria in the samples. Case histories contained information on high incidence of positive Mantoux test. Microbiologically the diagnosis was confirmed only in 7 adolescents. In children mycobacteria were not found.

摘要

采用聚合酶链反应(PCR)检测儿童和青少年临床样本中的分枝杆菌DNA。结果分别在23%和53%的病例中呈阳性,而标准微生物学方法未能显示样本中存在任何细菌。病历包含了结核菌素试验阳性高发生率的信息。微生物学诊断仅在7名青少年中得到证实。在儿童中未发现分枝杆菌。

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