在自动化HPGE - 1000仪器中，将厚度为0.5毫米的凝胶应用于电泳：分辨率提高。

Application of gels of 0.5 mm thickness to electrophoresis in the automated HPGE-1000 apparatus: improved resolution.

作者信息

Chang H T, Chrambach A

机构信息

Section on Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.

出版信息

Electrophoresis. 1996 Jan;17(1):80-3. doi: 10.1002/elps.1150170114.

DOI:10.1002/elps.1150170114

PMID:8907522

Abstract

Gel electrophoresis in commercial automated apparatus (HPGE-1000, LabIntelligence, Menlo Park, CA) is conventially conducted in gels of 3 mm thickness at about 15 V/cm. Since the intermittent scanning of the gel allows one to measure band width as a function of migration time, resolution may be evaluated quantitatively. Comparing the value of resolution between two proteins in electrophoresis on agarose gels of 0.5 and 3.0 mm thickness and at various field strengths, it was found that within the given Joule heat dissipation capacity of the apparatus, resolution between the proteins is improved when the gel thickness is reduced from 3.0 to 0.5 mm, which allows for an increase in field strength from 15 V/cm, conventional for that apparatus, to 45 V/cm.

摘要

在商用自动装置（HPGE - 1000，LabIntelligence，加利福尼亚州门洛帕克）中进行的凝胶电泳，通常是在厚度为3毫米的凝胶中以约15伏/厘米的电场强度进行。由于对凝胶的间歇扫描能够让人们测量条带宽度作为迁移时间的函数，因此可以对分辨率进行定量评估。比较在厚度为0.5毫米和3.0毫米的琼脂糖凝胶上以及在不同电场强度下进行电泳时两种蛋白质之间的分辨率值，发现在该装置给定的焦耳热耗散能力范围内，当凝胶厚度从3.0毫米减小到0.5毫米时，蛋白质之间的分辨率会提高，这使得电场强度能够从该装置常规的15伏/厘米增加到45伏/厘米。