Forsgren J, Samuelson A, Borrelli S, Christensson B, Jonasson J, Lindberg A A
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Huddinge Hospital, Sweden.
Acta Otolaryngol. 1996 Sep;116(5):766-73. doi: 10.3109/00016489609137922.
That nontypeable H. influenzae (NTHI) can reside intracellularly in human adenoid tissue has been suggested by use of in situ hybridization of a fluorescein labelled 16S rRNA-targeted oligonucleotide probe (FISH). Adenoid tissues from 43 children operated on in a clinically infection-free interval were investigated. FISH revealed H. influenzae in macrophage-like cells, located subepithelially in the crypts in all 43 adenoids. Furthermore, H. influenzae was detected in 22/22 adenoids using immunohistochemistry with the monoclonal antibody MAHI-3 recognizing a conserved H. influenzae LPS inner-core region. FISH and staining with monoclonal antibodies against immunophenotypic markers were performed simultaneously in order to characterize the cellular interrelations in this microenvironment. The findings of widespread presence of H. influenzae in cells of which some strongly expressed the CD14 marker of the monocyte/macrophage lineage may correspond to an important aspect of the colonization mechanisms whereby NTHI persists in the nasopharynx of children.
通过使用荧光素标记的靶向16S rRNA的寡核苷酸探针（FISH）进行原位杂交，提示不可分型流感嗜血杆菌（NTHI）能够在人腺样体组织细胞内生存。对43名处于临床无感染期的接受手术的儿童的腺样体组织进行了研究。FISH显示，在所有43个腺样体隐窝上皮下的巨噬细胞样细胞中发现了流感嗜血杆菌。此外，使用识别保守的流感嗜血杆菌LPS内核区域的单克隆抗体MAHI-3进行免疫组织化学检测，在22/22个腺样体中检测到了流感嗜血杆菌。同时进行FISH和针对免疫表型标志物的单克隆抗体染色，以表征这种微环境中的细胞间关系。在一些强烈表达单核细胞/巨噬细胞谱系CD14标志物的细胞中广泛存在流感嗜血杆菌，这一发现可能对应于NTHI在儿童鼻咽部持续存在的定植机制的一个重要方面。
