Riches P G, Sellwood S M, Harrap K R
Chem Biol Interact. 1977 Jul;18(1):11-22. doi: 10.1016/0009-2797(77)90137-5.
The ability of nuclei, isolated from Yoshida ascites sarcoma cells, to phosphorylate nuclear proteins in the presence of [gamma-32P]ATP has been investigated. Comparisons were made between a strain sensitive to the effects of the alkylating agent, chlorambucil, with a corresponding resistant strain both before and after drug-treatment of tumour-bearing animals. There was no gross quantitative differences between the drug-sensitive and-resistant untreated cells but treatment resulted in increased levels in the sensitive strain. Qualitative differences were seen before treatment in the phosphorylation pattern of the tris-saline-soluble nuclear sap fraction. The high molecular weight species in the fraction from sensitive cells showed phosphorylations which were absent, or present at very low levels, in the corresponding fraction from drug-resistant cells. Changes were observed in the tris-saline-soluble and non-histone protein fractions from drug-sensitive cells following treatment of tumour-bearing animals. Only minor alterations in patterns of phosphorylation were seen in fractions from drug-resistant cells.
对从吉田腹水肉瘤细胞中分离出的细胞核在[γ-32P]ATP存在下使核蛋白磷酸化的能力进行了研究。在对荷瘤动物进行药物治疗之前和之后，对一株对烷化剂苯丁酸氮芥敏感的菌株与相应的耐药菌株进行了比较。未经处理的药物敏感细胞和耐药细胞之间没有明显的定量差异，但药物处理导致敏感菌株中的水平升高。在处理之前，在三盐溶液可溶性核液部分的磷酸化模式中观察到了定性差异。来自敏感细胞的部分中的高分子量物质显示出磷酸化，而在来自耐药细胞的相应部分中不存在或仅以非常低的水平存在。在对荷瘤动物进行治疗后，观察到药物敏感细胞的三盐溶液可溶性和非组蛋白部分发生了变化。在耐药细胞的部分中仅观察到磷酸化模式的微小改变。
