人类核糖体蛋白L39和S27的一级结构及序列分析

Primary structures and sequence analysis of human ribosomal proteins L39 and S27.

作者信息

Tsui S K, Lee S M, Fung K P, Waye M M, Lee C Y

机构信息

Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.

出版信息

Biochem Mol Biol Int. 1996 Oct;40(3):611-6. doi: 10.1080/15216549600201203.

DOI:10.1080/15216549600201203

PMID:8908372

Abstract

We report here the primary structures and sequence analysis of the human ribosomal protein L39 (hRPL39) and S27 (hRPS27). The hRPL39 cDNA is 352 bp in size encoding a predicted protein of 51 amino acids. The region KRRHWRRTKL near the carboxyl end is conserved between human, rat, maize, C. elegans and yeast. The hRPS27 cDNA is 321 bp in size encoding a deduced protein of 84 amino acids. When the deduced amino acid sequence of hRPS27 was compared with that of rat ribosomal protein S27 and human metalloproteinstimulin-1 (MPS-1), identity levels of 96.4% and 100% were obtained respectively. A potential polyadenylation signal AACAAA is found in the MPS-1 cDNA but the more frequently used AATAAA sequence is present in the hRPS27 cDNA. The carboxyl-terminal cysteine arrangement in hRPS27 is similar to the family of C4 zinc finger DNA-binding proteins.

摘要

我们在此报告人类核糖体蛋白L39（hRPL39）和S27（hRPS27）的一级结构及序列分析。hRPL39 cDNA大小为352 bp，编码一个由51个氨基酸组成的预测蛋白。人、大鼠、玉米、秀丽隐杆线虫和酵母的羧基末端附近的KRRHWRRTKL区域是保守的。hRPS27 cDNA大小为321 bp，编码一个由84个氨基酸组成的推导蛋白。当将hRPS27的推导氨基酸序列与大鼠核糖体蛋白S27和人类金属蛋白酶刺激素-1（MPS-1）的序列进行比较时，同源性水平分别为96.4%和100%。在MPS-1 cDNA中发现了一个潜在的多聚腺苷酸化信号AACAAA，但hRPS27 cDNA中存在更常用的AATAAA序列。hRPS27的羧基末端半胱氨酸排列类似于C4锌指DNA结合蛋白家族。