Kang J H, Lee M K, Kim K L, Hahm K S
Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, Korea.
Biochem Mol Biol Int. 1996 Oct;40(3):617-27. doi: 10.1080/15216549600201213.
Synthetic pulmonary surfactants consisting of a mixture of phospholipids with synthetic peptides based on human and bovine surfactant-associated protein SP-B were prepared. These surfactants were analyzed for their biophysical activities by Wilhemly balance experiments and for their secondary structures by circular dichroism (CD) spectroscopy. Four synthetic peptides (SP-1, SP-2, SP-3, and SP-4) combined with the phospholipid mixture displayed significant surfactant properties. The CD spectra showed that the alpha-helical propensities of the peptides in SDS micelles were related to their surfactant activities. These results suggested that the several truncated peptides originated from SP-B protein, when appropriately recombined with phospholipids, could be used as an effective synthetic surfactant for clinical use.
制备了由磷脂与基于人和牛表面活性剂相关蛋白SP - B的合成肽混合物组成的合成肺表面活性剂。通过威尔海姆天平实验分析了这些表面活性剂的生物物理活性，并通过圆二色性（CD）光谱分析了它们的二级结构。四种与磷脂混合物结合的合成肽（SP - 1、SP - 2、SP - 3和SP - 4）表现出显著的表面活性剂特性。CD光谱表明，肽在SDS胶束中的α - 螺旋倾向与其表面活性剂活性相关。这些结果表明，几种源自SP - B蛋白的截短肽，当与磷脂适当重组时，可作为有效的合成表面活性剂用于临床。
