Ohashi N, Koyama Y, Urakami H, Fukuhara M, Tamura A, Kawamori F, Yamamoto S, Kasuya S, Yoshimura K
Department of Microbiology, Niigata College of Pharmacy, Japan.
Microbiol Immunol. 1996;40(9):627-38. doi: 10.1111/j.1348-0421.1996.tb01120.x.
A total of 40 strains of Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, and 6 prototype strains of antigenic variants) were examined for classification based on the reactivities with type-specific monoclonal antibodies in indirect immunofluorescence tests, and on the restriction fragment length polymorphism of a polymerase chain reaction (PCR)-amplified 56-kilodalton type-specific antigenic protein gene. By these methods, several antigenic and genotypic variants were found among the strains, and these variants were classified into types and further into subtypes. These results suggest that there are many variants in O. tsutsugamushi, and the methods used here seem to be useful for the systematic classification of the numerous variants. A strain which may be a new type distinguishable from those identified previously was also found in this study. Furthermore, variety in the degree of pathogenicity in mice related to type and/or subtype classification were observed.
总共40株恙虫病东方体（34株分离自日本患者和恙螨，以及6株抗原变异原型株）基于间接免疫荧光试验中与型特异性单克隆抗体的反应性，以及聚合酶链反应（PCR）扩增的56千道尔顿型特异性抗原蛋白基因的限制性片段长度多态性进行分类检测。通过这些方法，在菌株中发现了几种抗原和基因型变异体，并将这些变异体分为不同类型，进而再分为亚型。这些结果表明恙虫病东方体存在许多变异体，此处使用的方法似乎有助于对众多变异体进行系统分类。在本研究中还发现了一株可能是与先前鉴定的菌株不同的新型菌株。此外，观察到与类型和/或亚型分类相关的小鼠致病性程度存在差异。
