采用简单的散射比浊法测定血清或尿液中的溶菌酶。
Lysozyme determined in serum or urine by a simple nephelometric method.
作者信息
Borgen J, Romslo I
出版信息
Clin Chem. 1977 Sep;23(9):1599-601.
PMID:890902
Abstract
We describe a nephelometric procedure for determination of lysozyme. A quantitative result is obtained in 2 to 3 min with 50 microliter of serum or urine. At a lysozyme concentration of 12.0 mg/liter, between-day precision was 3.4% and the analytical recovery 96.5-105.5%. The values obtained were a linear function of enzyme concentration below 50 mg/liter, and they agreed with those obtained by absorptiometric determination of the decrease in turbidity of a suspension of Micrococcus lysodeikticus.
摘要
我们描述了一种用于测定溶菌酶的比浊法。用50微升血清或尿液在2至3分钟内可获得定量结果。在溶菌酶浓度为12.0毫克/升时，日间精密度为3.4%，分析回收率为96.5 - 105.5%。在低于50毫克/升的酶浓度下，所获得的值是酶浓度的线性函数，并且与通过比色法测定溶壁微球菌悬液浊度降低所获得的值一致。
相似文献
1
Lysozyme determined in serum or urine by a simple nephelometric method.采用简单的散射比浊法测定血清或尿液中的溶菌酶。
Clin Chem. 1977 Sep;23(9):1599-601.
2
Serum lysozyme: evaluation of a nephelometric assay.血清溶菌酶：散射比浊法测定的评估
Scand J Clin Lab Invest. 1981 Jun;41(4):329-32. doi: 10.3109/00365518109092053.
3
Development of a microparticle-enhanced nephelometric immunoassay for quantitation of human lysozyme in pleural effusion and plasma.一种用于定量检测胸腔积液和血浆中人类溶菌酶的微粒增强散射比浊免疫分析法的开发。
J Clin Lab Anal. 1999;13(6):301-7. doi: 10.1002/(sici)1098-2825(1999)13:6<301::aid-jcla9>3.0.co;2-3.
4
A method for automatic recording of serum lysozyme activity with the fragiligraph.
Experientia. 1976 Feb 15;32(2):257-9. doi: 10.1007/BF01937797.
5
[Determination of lysozyme in biological fluids by a semi-automatic kinetic technique. Discussion of the method (author's transl)].[用半自动动力学技术测定生物体液中的溶菌酶。方法讨论（作者译）]
Clin Chim Acta. 1979 Apr 2;93(1):51-60. doi: 10.1016/0009-8981(79)90244-4.
6
Turbidimetric determination of lysozyme with Micrococcus lysodeikticus cells: reexamination of reaction conditions.用溶壁微球菌细胞比浊法测定溶菌酶：反应条件的重新审视
Anal Biochem. 1983 Jan;128(1):77-85. doi: 10.1016/0003-2697(83)90347-0.
7
Determination of lysozyme activity by a fluorescence technique in comparison with the classical turbidity assay.通过荧光技术与经典比浊法测定溶菌酶活性。
Pharmazie. 2008 Jun;63(6):415-9.
8
Immunochemical determination of human lysozyme by laser nephelometry.
Clin Chem. 1977 Jun;23(6):967-70.
9
[Analysis of methods for optimizing lysozyme determination].[溶菌酶测定方法的优化分析]
Allerg Immunol (Leipz). 1982;28(4):251-9.
10
Improved turbidimetric assay for lysozyme in urine.
Clin Chem. 1983 Aug;29(8):1488-93.
引用本文的文献
1
Development of a microparticle-enhanced nephelometric immunoassay for quantitation of human lysozyme in pleural effusion and plasma.一种用于定量检测胸腔积液和血浆中人类溶菌酶的微粒增强散射比浊免疫分析法的开发。
J Clin Lab Anal. 1999;13(6):301-7. doi: 10.1002/(sici)1098-2825(1999)13:6<301::aid-jcla9>3.0.co;2-3.
Zotero 插件