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[用半自动动力学技术测定生物体液中的溶菌酶。方法讨论(作者译)]

[Determination of lysozyme in biological fluids by a semi-automatic kinetic technique. Discussion of the method (author's transl)].

作者信息

Plouet J, Madec Y

出版信息

Clin Chim Acta. 1979 Apr 2;93(1):51-60. doi: 10.1016/0009-8981(79)90244-4.

Abstract

A rapid and semi-automatic determination of lysozyme in biological fluids using kinetic analysis and turbidimetry is described and compared to other commonly used techniques. The specificity of the method is satisfactory while that of clarification of a gel medium is apparently not. Normal values and standard errors for plasma, urine, and leucocytes are given. A standardised expression of lysozyme activity is proposed and discussed. The importance of the means by which the blood sample is collected and prepared is underlined: plasma, decanted soon after collection, is preferable to serum.

摘要

描述了一种使用动力学分析和比浊法快速半自动测定生物体液中溶菌酶的方法,并与其他常用技术进行了比较。该方法的特异性令人满意,而凝胶介质澄清法的特异性显然不令人满意。给出了血浆、尿液和白细胞的正常值及标准误差。提出并讨论了溶菌酶活性的标准化表达。强调了采集和制备血样的方式的重要性:采集后不久倾析出的血浆比血清更可取。

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