使用碘化示踪剂对苯妥英进行放射免疫测定的评估。

The evaluation of a radioimmunoassay for diphenylhydantoin using an iodinated tracer.

作者信息

Paxton J W, Rowell F J, Ratcliffe J G

出版信息

Clin Chim Acta. 1977 Aug 15;79(1):81-92. doi: 10.1016/0009-8981(77)90464-8.

Abstract

Radioimmunoassays (RIA) for diphenylhydantoin (DPH) are described using a rabbit antiserum to the immunogen, diphenylhydantoin-3-omega-valerate bovine serum albumin, and DPH labelled with carbon-14 (14C), tritium (3H) or iodine-125 (125I). The [125I]DPH label is prepared by iodination of DPH-3-N-ethyl-acetamido-tyrosine ethyl ester, and is stable for several months. The radioimmunoassays are rapid, precise, specific and sensitive requiring only 50 microliter serum for duplicate analyses. Serum DPH levels measured by the three RIA methods correlate well with each other (3H vs. 14C, r = 0.96 : 3H vs. 125I, r = 0.97) and with values obtained by a conventional gas chromatographic method (125I RIA vs. GC, r = 0.95). However, RIA employing the iodinated DPH tracer has significant practical advantages over the other methods and is well suited to large scale clinical application.

摘要

本文描述了用于测定苯妥英（DPH）的放射免疫分析法（RIA），该方法使用针对免疫原苯妥英 - 3 - ω - 戊酸牛血清白蛋白的兔抗血清，以及用碳 - 14（14C）、氚（3H）或碘 - 125（125I）标记的DPH。[125I]DPH标记物通过对DPH - 3 - N - 乙基 - 乙酰氨基 - 酪氨酸乙酯进行碘化制备，并且在几个月内保持稳定。这些放射免疫分析法快速、精确、特异且灵敏，重复分析仅需50微升血清。通过三种RIA方法测得的血清DPH水平彼此之间相关性良好（3H与14C相比，r = 0.96；3H与125I相比，r = 0.97），并且与通过传统气相色谱法获得的值相关性良好（125I RIA与GC相比，r = 0.95）。然而，采用碘化DPH示踪剂的RIA相对于其他方法具有显著的实际优势，非常适合大规模临床应用。