使用碘化示踪剂对吩噻嗪类和硫杂蒽类进行放射免疫分析的评估。

The evaluation of a radioimmunoassay for phenothiazines and thioxanthenes using an iodinated tracer.

作者信息

Rowell F J, Hui S M, Paxton J W

出版信息

J Immunol Methods. 1979;31(1-2):159-66. doi: 10.1016/0022-1759(79)90296-5.

Abstract

Antisera have been raised in rabbits to the immogen 2-trifluoromethylphenothiazine-10-beta-propionate bovine serum albumin. An [125I]-labelled tyrosine methyl ester derivative of the immunogen precursor has been synthesised and used with the antisera to develop a simple, precise and sensitive radioimmunoassay for phenothiazines and thioxanthenes bearing 2-trifluoromethyl substituents. The assay can detect 0.4 ng/ml of fluphenazine, trifluorperazine or (Z)-flupenthixol in 100 microliter of human serum without interference from their sulphoxide or 7-hydroxylated metabolites. An acceptable correlation between this assay and an established fluphenazine radioimmunoassay using commercial [3H]fluphenazine has been obtained for plasma samples.

摘要

已在兔体内制备了针对免疫原2-三氟甲基吩噻嗪-10-β-丙酸酯牛血清白蛋白的抗血清。已合成免疫原前体的[125I]标记酪氨酸甲酯衍生物，并将其与抗血清一起用于开发一种简单、精确且灵敏的放射免疫分析法，用于检测带有2-三氟甲基取代基的吩噻嗪类和噻吨类药物。该分析方法可在100微升人血清中检测出0.4纳克/毫升的氟奋乃静、三氟拉嗪或（Z）-氟哌噻吨，且不受其亚砜或7-羟基化代谢物的干扰。对于血浆样本，该分析方法与使用商业[3H]氟奋乃静的既定氟奋乃静放射免疫分析法之间已获得可接受的相关性。