通过溴脱氧尿苷和增殖细胞核抗原免疫组织化学评估下颌成釉细胞纤维牙瘤中的细胞动力学：病例报告

Cell kinetics in mandibular ameloblastic fibro-odontoma evaluated by bromodeoxyuridine and proliferating cell nuclear antigen immunohistochemistry: case report.

作者信息

Sekine J, Kitamura A, Ueno K, Sano K, Inokuchi T, Takahashi H, Okabe H

机构信息

Second Department of Oral and Maxillofacial Surgery, Nagasaki University, School of Dentistry, Japan.

出版信息

Br J Oral Maxillofac Surg. 1996 Oct;34(5):450-3. doi: 10.1016/s0266-4356(96)90106-0.

DOI:10.1016/s0266-4356(96)90106-0

PMID:8909741

Abstract

A case of ameloblastic fibro-odontoma in a 10-year-old Japanese male patient is presented. Bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA) immunohistochemistry were applied to investigate the cell kinetics of this lesion. The tumour proliferation activity was individually evaluated through the labelling index (LI) at the epithelial and mesenchymal components. The LI of BrdU was 0.9% in the epithelial and 2.1% in the mesenchymal component, while the LI of PCNA was 2.2% in the epithelial and 7.7% in the mesenchymal component. These immunohistochemical investigations suggest that the mesenchymal component was more proliferative than the epithelial component. Such an immunohistochemical investigation may be useful in evaluating the proliferative potential of odontogenic tumours.

摘要

本文报告一例10岁日本男性患者的成釉细胞纤维牙瘤病例。应用溴脱氧尿苷（BrdU）和增殖细胞核抗原（PCNA）免疫组织化学方法研究该病变的细胞动力学。通过上皮和间充质成分的标记指数（LI）分别评估肿瘤增殖活性。BrdU的LI在上皮成分中为0.9%，在间充质成分中为2.1%，而PCNA的LI在上皮成分中为2.2%，在间充质成分中为7.7%。这些免疫组织化学研究表明，间充质成分比上皮成分增殖性更强。这种免疫组织化学研究可能有助于评估牙源性肿瘤的增殖潜能。