de Wael J, Raaymakers C E, Endeman H J
Clin Chim Acta. 1977 Sep 1;79(2):465-70. doi: 10.1016/0009-8981(77)90443-0.
To determine total fecal bile acids, these are extracted with diethyl ether after boiling with a solution of potassium hydroxide in ethanediol. After evaporating the ether and dissolving the residue in methanol, the bile acids are directly determined with 3 alpha-hydroxysteroid dehydrogenase. Values for 9 normals are given.