Díaz F, Villena A, Requena V, González P, Peláez A, Pérez de Vargas I
Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, Spain.
Mech Ageing Dev. 1996 Oct 4;91(1):47-54. doi: 10.1016/0047-6374(96)01777-0.
We carried out a quantitative histochemical study of the enzyme cytochrome oxidase (CO) in neurons of the dorsal lateral geniculate nucleus (dLGN) of male Wistar rats aged 3, 18, 24 and 28 months. The results show that the activity of cytochrome oxidase decreases significantly between 24 and 28 months. We also checked whether a correlation existed between neuronal size and enzymatic activity. Low correlation coefficients were obtained which were between 0.4139 at 3 months 0.2092 at 28 months. Nevertheless, we observed a certain relationship between both parameters, and therefore we classified the neurons as light, moderate and dark according to their optical density, which correlates with enzyme cytochrome oxidase activity, and as small, medium and large depending on their size. We found that light neurons were scarcely represented in the dLGN. At the age of 3 months, the most frequent neurons were moderate, medium-size ones, and dark, small ones. The population of moderate neurons increased with age, reaching 74.5% at the 28th month, 52.2% of which corresponded to medium-size neurons. In the same group dark neurons decreased, falling to a total of 15.3% made up of medium and large-size ones. These results could be interpreted as reflecting a decrease in the bioenergetic competence of the neurons of this nucleus in old age.
我们对3、18、24和28月龄雄性Wistar大鼠背外侧膝状核（dLGN）神经元中的细胞色素氧化酶（CO）进行了定量组织化学研究。结果表明，细胞色素氧化酶的活性在24至28月龄之间显著下降。我们还检查了神经元大小与酶活性之间是否存在相关性。得到的相关系数较低，3月龄时为0.4139，28月龄时为0.2092。然而，我们观察到这两个参数之间存在一定的关系，因此我们根据与细胞色素氧化酶活性相关的光密度将神经元分为浅、中、深三类，并根据其大小分为小、中、大三类。我们发现浅神经元在dLGN中很少见。3月龄时，最常见的神经元是中等大小的中等神经元和小的深色神经元。中等神经元的数量随年龄增加，在第28个月时达到74.5%，其中52.2%为中等大小的神经元。在同一组中，深色神经元减少，总数降至15.3%，由中等和大尺寸的神经元组成。这些结果可以解释为反映了该核团神经元在老年时生物能量能力的下降。
